13154 1110018G07Rik Status: Cancelled
| Gene | 1110018G07Rik |
| Chromosome | 12 |
| Deletion Start | 84,943,344 |
| Deletion End | 84,920,385 |
| Deletion Size | 22,960 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 84,942,863 | 84,942,881 | TGCCTGGGCTTCGTGATAG |
| TUP | 84,942,837 | 84,942,862 | ACAACAGGGTCAGCAGACTGGTCTGT |
| TUR | 84,942,775 | 84,942,798 | GAGTATGTGGAAGGAGACCGTATG |
| TDF | 84,921,032 | 84,921,054 | GAAGTAAGCAGAGCCAAGCAATC |
| TDP | 84,921,004 | 84,921,028 | TGGTGATTACCCGCTGATCCTGTGA |
| TDR | 84,920,947 | 84,920,967 | GCTCCCAGTTACAGCACTATG |
PCR Primers
| Primer | Sequence |
| SU | AATCAAGTGGCTGCCTTCTG |
| SD | TGCACTTCTCCTGACCACTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 107 bp | + | + | - |
| TDF | TDR | 108 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
