| Gene | Ptcd3 |
| Chromosome | 6 |
| Deletion Start | 71,908,737 |
| Deletion End | 71,881,146 |
| Deletion Size | 27,592 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 71,908,616 | 71,908,637 | GCAGGTAAGAAGACCCAGAGTC |
| TUP | 71,908,586 | 71,908,611 | TGGTCTTGCATTTCCCTGAGAGTCGT |
| TUR | 71,908,516 | 71,908,537 | GCTACCTCGTGTTACCCTTGAG |
| TDF | 71,881,607 | 71,881,626 | CATCCGGTAGCACTGCTCTG |
| TDP | 71,881,577 | 71,881,602 | TTGAGGTTGTAAAGCTGGCAAGTGCC |
| TDR | 71,881,536 | 71,881,558 | ACTACTCTTTGGGCAAGGCTCTC |
PCR Primers
| Primer | Sequence |
| SU | GAGTCTTGTAGCGCAGAGCC |
| SD | TCTAGGCCAAAGGACCACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 122 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13163A-B11 | VGB6 | C57BL/6NTac | |
| 13163A-D8 | VGB6 | C57BL/6NTac | |
| 13163A-E4 | VGB6 | C57BL/6NTac | |
| 13163A-F7 | VGB6 | C57BL/6NTac | |
| 13163A-C9 | VGB6 | C57BL/6NTac | |
| 13163A-A7 | VGB6 | C57BL/6NTac | |
