13168 Ptplb Status: ES cell colonies screened / QC no positives
| Gene | Ptplb |
| Chromosome | 16 |
| Deletion Start | 35,022,484 |
| Deletion End | 35,048,733 |
| Deletion Size | 26,250 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 35,022,885 | 35,022,908 | TGGCTGCGGAGATCATTCATTTAG |
| TUP | 35,022,910 | 35,022,931 | CGGCCGCTTTCAAAACCCAGGC |
| TUR | 35,022,962 | 35,022,980 | GGCGGAAAACTTCGACCAC |
| TDF | 35,048,335 | 35,048,353 | GCAGCAGTCGCCAACAAAG |
| TDP | 35,048,358 | 35,048,383 | TACTAACGCCACACTGTGTAGCCTGT |
| TDR | 35,048,401 | 35,048,420 | CACCAGGCATCTCCGTAATC |
PCR Primers
| Primer | Sequence |
| SU | GATCCAGCAGGAAGAGATGG |
| SD | TTCTCCAGCTCAGCACAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 96 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
