13171   0610007L01Rik     Status: ES cell colonies screened / QC positives

Gene0610007L01Rik
Chromosome5
Deletion Start130,705,341
Deletion End130,717,205
Deletion Size11,865
Genome Build37
MGI IDMGI:1918917
NCBI Gene ID71667
BAC ID453A10
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 5

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUFTGCTGGGATTATGCGTTGTG
TUPCACCACATCTGGACTGTTCTTTCTGAGACA
TURAGGGCAGCTTGGGCTTCAG
TDFCACAGAAGTCATGCAGCAGAG
TDPACCTATCAGGGCCAACTAACTAGTGGCTA
TDRCACTGTACTAACCAAGCCACATCC

PCR Primers

PrimerSequence
SUTTCTAGTCTTCCAAATGAGCTC
SDAAGAACCACAGCAAACCTCG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTCATTCTCAGTATTGTTTTGCC
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
13171A-C5VGB6C57BL/6NTac
13171A-H6VGB6C57BL/6NTac
13171A-B11VGB6C57BL/6NTac
13171A-G4VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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