13173 Sars2 Status: ES cell colonies screened / QC no positives
| Gene | Sars2 |
| Chromosome | 7 |
| Deletion Start | 28,742,019 |
| Deletion End | 28,753,630 |
| Deletion Size | 11,612 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 28,742,090 | 28,742,107 | GTGCAGCCAGAACCCAAG |
| TUP | 28,742,114 | 28,742,138 | TTTCGCTACGGAGAAACGAGTCCGG |
| TUR | 28,742,168 | 28,742,189 | CAAGTAGGGAAGCTCGCTGTAG |
| TDF | 28,753,404 | 28,753,423 | GCATCCTAACAACCGCTCTG |
| TDP | 28,753,424 | 28,753,446 | TCGCCAGGATGGTTCAGTCCTCG |
| TDR | 28,753,526 | 28,753,545 | TGGGCTTCTGAGGCTGATTG |
PCR Primers
| Primer | Sequence |
| SU | ATAACTGCACCCACCTCACC |
| SD | GAGGAAGGGAGAGATCCTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 142 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
