13188 5033411D12Rik Status: Cancelled
| Gene | 5033411D12Rik |
| Chromosome | 13 |
| Deletion Start | 17,694,729 |
| Deletion End | 17,662,228 |
| Deletion Size | 32,502 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 17,693,976 | 17,693,997 | TGGTTGTGAGCTACCTTGTGAG |
| TUP | 17,693,952 | 17,693,975 | TGCTGGGAACTGAGCCAAATCCTC |
| TUR | 17,693,899 | 17,693,917 | GCCAGGCGCTGAAGAGATG |
| TDF | 17,662,955 | 17,662,978 | AGAAAGTGGAGGTCTGGTAAGTTG |
| TDP | 17,662,914 | 17,662,936 | TCCCAGCGGATGAGGATCCACTA |
| TDR | 17,662,853 | 17,662,870 | GCAAACCGACAGGCTCAC |
PCR Primers
| Primer | Sequence |
| SU | ATCAGGCCTTATTGGCTGTG |
| SD | AAAACACTAGTGAGAAACAAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 126 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
