13203 Atp5j2 Status: ES cell colonies screened / QC one positive
| Gene | Atp5j2 |
| Chromosome | 5 |
| Deletion Start | 145,191,512 |
| Deletion End | 145,183,767 |
| Deletion Size | 7,746 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 145,190,612 | 145,190,629 | GCTGCCACAGTCGTGATG |
| TUP | 145,190,575 | 145,190,603 | TCTATCTCTGAGTGCTAGGATTACAGGCC |
| TUR | 145,190,527 | 145,190,548 | CCCAGGCTGAGATAAAGAACAC |
| TDF | 145,184,469 | 145,184,490 | CGTGGCTTTGCTAGTTCGATAC |
| TDP | 145,184,438 | 145,184,462 | CCACCCTGTTAGCAGTGGAGCTCTG |
| TDR | 145,184,407 | 145,184,427 | AGGTGGAAGAACGCACAGGTC |
PCR Primers
| Primer | Sequence |
| SU | TAAGCAGGTGTCAGCTGTGG |
| SD | AGCACCTCTAGACAGCCACAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 103 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
