13206 B4galt6 Status: Cancelled
| Gene | B4galt6 |
| Chromosome | 18 |
| Deletion Start | 20,745,464 |
| Deletion End | 20,720,036 |
| Deletion Size | 25,429 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 20,744,967 | 20,744,988 | CAACTGCGAGGAAGGTCAAGAG |
| TUP | 20,744,938 | 20,744,964 | TGCATGCTGGTATTGCAGGTCAACAGA |
| TUR | 20,744,898 | 20,744,921 | GGGTTTCAACAGCTACAATGTCTC |
| TDF | 20,720,815 | 20,720,834 | AAACCGACTGTTGCGCTCTG |
| TDP | 20,720,789 | 20,720,813 | AGGCGAGCCTTTGCTGGCACGCTAT |
| TDR | 20,720,758 | 20,720,779 | CATCAGGTTCCTCCACACTCAG |
PCR Primers
| Primer | Sequence |
| SU | GGGACTCGCCTCTTGGAC |
| SD | CTGAATGGGAGAAACACAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
