| Gene | Dpt |
| Chromosome | 1 |
| Deletion Start | 164,796,735 |
| Deletion End | 164,823,246 |
| Deletion Size | 26,512 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 164,796,738 | 164,796,759 | CTCACTCTTCTGTGGGTTCTTC |
| TUP | 164,796,760 | 164,796,780 | TGCCACTGGTGACCACAGCCT |
| TUR | 164,796,825 | 164,796,846 | CCCATCCATCATCACCGTAGTC |
| TDF | 164,822,454 | 164,822,471 | TGGGCACATTGGAAGCAC |
| TDP | 164,822,473 | 164,822,498 | AGGGACTTCACAATGACAATGGTCGC |
| TDR | 164,822,514 | 164,822,534 | AGCACTCCTGCGGTCTGATAC |
PCR Primers
| Primer | Sequence |
| SU | CCCTCTCCGTGAAGTGATTC |
| SD | TAGTGCGAATCACAGCAAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 109 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13207A-D10 | VGB6 | C57BL/6NTac | |
| 13207A-E5 | VGB6 | C57BL/6NTac | |
| 13207A-F1 | VGB6 | C57BL/6NTac | |
| 13207A-E3 | VGB6 | C57BL/6NTac | |
