13215 Crb1 Status: Cancelled
| Gene | Crb1 |
| Chromosome | 1 |
| Deletion Start | 139,337,593 |
| Deletion End | 139,314,576 |
| Deletion Size | 23,018 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 139,337,356 | 139,337,375 | AGCGGGCTGAACTGTGAAAC |
| TUP | 139,337,332 | 139,337,355 | TGCCACCAATTCCTGTGGAGGGAA |
| TUR | 139,337,285 | 139,337,305 | CAGGGTGCTCAGGGTCTTTAC |
| TDF | 139,314,832 | 139,314,849 | TAATTGGCACCGGCACAG |
| TDP | 139,314,801 | 139,314,827 | CAGCCTCTTCTAAGACATGTGCCTGGC |
| TDR | 139,314,774 | 139,314,796 | GAAGCTGCTTAGTCAAAGCTCAG |
PCR Primers
| Primer | Sequence |
| SU | AAACTTCTGGATAAGTGAAGAG |
| SD | GTTTACTTCTAGGCTGAAGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
