| Gene | Agpat6 |
| Chromosome | 8 |
| Deletion Start | 23,190,877 |
| Deletion End | 23,174,515 |
| Deletion Size | 16,363 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 23,190,830 | 23,190,851 | GATTGTCAACCTCCTGGGTATC |
| TUP | 23,190,806 | 23,190,829 | TCCCTGACCGTCCTCTTCACCCTG |
| TUR | 23,190,772 | 23,190,791 | CAAAGATGGCAGGGACGATG |
| TDF | 23,174,957 | 23,174,978 | GTCTCTTGCCACCACTTCTTTG |
| TDP | 23,174,931 | 23,174,956 | TGCCATTGTAGAGTGAAATCGGGCAA |
| TDR | 23,174,906 | 23,174,929 | CTGCTTGTTCACGTCTTTATTCTC |
PCR Primers
| Primer | Sequence |
| SU | GGTGCTGTTCCAGCCTACTC |
| SD | CATCCTCTCAGGGCAAGAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13221A-A3 | VGB6 | C57BL/6NTac | |
| 13221A-A5 | VGB6 | C57BL/6NTac | |
| 13221A-E3 | VGB6 | C57BL/6NTac | |
| 13221A-B10 | VGB6 | C57BL/6NTac | |
| 13221A-E1 | VGB6 | C57BL/6NTac | |
| 13221A-A10 | VGB6 | C57BL/6NTac | |
