| Gene | Mreg |
| Chromosome | 1 |
| Deletion Start | 72,212,108 |
| Deletion End | 72,192,052 |
| Deletion Size | 20,057 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 72,211,250 | 72,211,270 | CGAATGAGGTCCCACCAAGTC |
| TUP | 72,211,216 | 72,211,245 | CCCAGGGAACAGAATTGAAATTAGGGCACA |
| TUR | 72,211,171 | 72,211,189 | GGCTTCTGCGCTTGTTCAC |
| TDF | 72,192,148 | 72,192,171 | ACTCTGGAGAGGGATGATGAGAAG |
| TDP | 72,192,118 | 72,192,142 | ATGGAGCATGCCTCATGACGTGTCC |
| TDR | 72,192,054 | 72,192,075 | TGGTCTGCTGATTACGAATGAC |
PCR Primers
| Primer | Sequence |
| SU | TCGCAGGACGCATGCGCT |
| SD | TGAGCTCTTGGGTATCAAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 118 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13225A-A10 | VGB6 | C57BL/6NTac | |
| 13225A-D1 | VGB6 | C57BL/6NTac | |
| 13225A-H11 | VGB6 | C57BL/6NTac | |
