13236 Gpr1 Status: Cancelled
| Gene | Gpr1 |
| Chromosome | 1 |
| Deletion Start | 63,184,071 |
| Deletion End | 63,182,947 |
| Deletion Size | 1,125 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 63,183,864 | 63,183,886 | GGGATTCAAGTGGAAGAAGACAG |
| TUP | 63,183,836 | 63,183,863 | TCACCACTCTTTGGTTCCTCAATCTGGC |
| TUR | 63,183,777 | 63,183,796 | TCAAGGCCACGTAGGAAATG |
| TDF | 63,183,175 | 63,183,199 | CCTCAATAGCTGCTTGAATCCCATC |
| TDP | 63,183,138 | 63,183,165 | CTAATAAGCAAGACGTTCCAAGCCCGCT |
| TDR | 63,183,100 | 63,183,122 | CAGCGAACGCTTTAGTACCTCAG |
PCR Primers
| Primer | Sequence |
| SU | TCCTTTAATGCACCATAATGGAC |
| SD | AAACACATGAACATGCACGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 110 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
