13253 Cnga4 Status: ES cell colonies screened / QC no positives
| Gene | Cnga4 |
| Chromosome | 7 |
| Deletion Start | 105,404,695 |
| Deletion End | 105,408,153 |
| Deletion Size | 3,459 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 105,405,492 | 105,405,513 | ACAGGGCATCCTGGTGGTAGAC |
| TUP | 105,405,516 | 105,405,543 | AAGCATGATCGCCAGTCGCTATGTACGC |
| TUR | 105,405,584 | 105,405,601 | GCACGTAGGCTGCATCTG |
| TDF | 105,407,661 | 105,407,681 | GGAAACCGACGAACAGCTAAC |
| TDP | 105,407,683 | 105,407,708 | TCAAGAGCCTGGGCTATTCAGACCTC |
| TDR | 105,407,720 | 105,407,741 | CACCTCCCGTAGATCCTCCTTG |
PCR Primers
| Primer | Sequence |
| SU | TAGGCCCAACAGAGAACCTG |
| SD | CTGCCTTCTTTCCCTGTAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 110 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
