13255 Rtn4rl2 Status: Cancelled
| Gene | Rtn4rl2 |
| Chromosome | 2 |
| Deletion Start | 84,886,689 |
| Deletion End | 84,871,945 |
| Deletion Size | 14,745 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 84,886,324 | 84,886,342 | AGCGCCGACTCAGCAACTC |
| TUP | 84,886,299 | 84,886,321 | CGTGCCCGGTGTCTCAGCACTTT |
| TUR | 84,886,265 | 84,886,286 | CCTACCCACATGGCTTGTCTTC |
| TDF | 84,872,833 | 84,872,855 | GCAGGAGATCCTCTTGGTTACAC |
| TDP | 84,872,804 | 84,872,829 | TGGGATACGATGCATGAGCCCTTTCC |
| TDR | 84,872,735 | 84,872,753 | ACGGTCGGGAACTCCACTC |
PCR Primers
| Primer | Sequence |
| SU | ACCGACCGACTAACCGACT |
| SD | AGCTTTCAAAGGCTCTGCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 121 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
