13266 Gfral Status: Cancelled
| Gene | Gfral |
| Chromosome | 9 |
| Deletion Start | 76,213,459 |
| Deletion End | 76,193,209 |
| Deletion Size | 20,251 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 76,212,959 | 76,212,978 | AGACCAAAGATGCTGCGTTC |
| TUP | 76,212,932 | 76,212,958 | TGCCTCTTGAGTAACATTCATGGCCCA |
| TUR | 76,212,909 | 76,212,931 | GAGCTAAGCCCACATTTTACCTG |
| TDF | 76,193,338 | 76,193,362 | CAAGACCTTACTTGTTCTGGGAGTG |
| TDP | 76,193,314 | 76,193,337 | AGAGCTGCAGGGCTGCCTTCCTAG |
| TDR | 76,193,289 | 76,193,309 | ACTTGCAGGACTGTCCCAAAG |
PCR Primers
| Primer | Sequence |
| SU | GTGTTCTAGTGGATGTGCGG |
| SD | AAGGCAAAACCGGAACATAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
