| Gene | Bgn |
| Chromosome | X |
| Deletion Start | 73,490,793 |
| Deletion End | 73,494,857 |
| Deletion Size | 4,065 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 73,490,910 | 73,490,932 | GCTTCAGGTTCAGACACCACTTC |
| TUP | 73,490,940 | 73,490,964 | CCCGACCTGGACTCTGTCACACCTA |
| TUR | 73,490,969 | 73,490,988 | CGAAAGGACACATGGCACTG |
| TDF | 73,494,727 | 73,494,745 | AGTGCAGCCTGCCACCTTC |
| TDP | 73,494,746 | 73,494,767 | CGCTGCGTTACTGACCGCCTGG |
| TDR | 73,494,793 | 73,494,810 | GGCAACCACTGCCTCTAC |
PCR Primers
| Primer | Sequence |
| SU | ATTGCCCAAATGAAACCAAG |
| SD | AGGGTTGGAGAGAGGGAAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13274A-A2 | VGB6 | C57BL/6NTac | |
| 13274A-A4 | VGB6 | C57BL/6NTac | |
| 13274A-B2 | VGB6 | C57BL/6NTac | |
| 13274A-C7 | VGB6 | C57BL/6NTac | |
| 13274A-C8 | VGB6 | C57BL/6NTac | |
| 13274A-D2 | VGB6 | C57BL/6NTac | |
