13288 Eng Status: Cancelled
| Gene | Eng |
| Chromosome | 2 |
| Deletion Start | 32,646,961 |
| Deletion End | 32,681,654 |
| Deletion Size | 34,694 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 32,647,273 | 32,647,295 | CCTTCCTGCTGTGTTCAGCTATC |
| TUP | 32,647,299 | 32,647,324 | AAGGCCAGGAGCTACACTGCTATGCA |
| TUR | 32,647,350 | 32,647,372 | CAAATGGGAAACTCAAGGCTCAG |
| TDF | 32,681,043 | 32,681,065 | ACCGAACTAGATGAAGAGACCTC |
| TDP | 32,681,069 | 32,681,093 | CCAGAGAAGTCGAGTCACTGACCCA |
| TDR | 32,681,105 | 32,681,125 | GGCCTTGGTCACGGGTATTAG |
PCR Primers
| Primer | Sequence |
| SU | GAAAGAGCCCAGTGACTCTT |
| SD | GCTCCCTCAGCTTCTGTTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
