13290 Fn1 Status: Cancelled
| Gene | Fn1 |
| Chromosome | 1 |
| Deletion Start | 71,652,979 |
| Deletion End | 71,624,084 |
| Deletion Size | 28,896 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 71,652,888 | 71,652,907 | GAAGCCGGGAAGAGCAAGAG |
| TUP | 71,652,864 | 71,652,886 | CAGGCTCAGCAAATCGTGCAGCC |
| TUR | 71,652,810 | 71,652,831 | GAACCAGACTGTCGGTACTCAC |
| TDF | 71,624,491 | 71,624,513 | ACACTATCCTGAGTCTCCCAGAG |
| TDP | 71,624,466 | 71,624,488 | CCACGGTATCCAGGGCTGTGGCT |
| TDR | 71,624,425 | 71,624,443 | GCATCCCAAGCTCCCTTAC |
PCR Primers
| Primer | Sequence |
| SU | GACGTCACCCGGACTCTG |
| SD | TCCTGACTCATAGTGTTAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
