13292 Gabra3 Status: Cancelled
| Gene | Gabra3 |
| Chromosome | X |
| Deletion Start | 72,552,813 |
| Deletion End | 72,540,070 |
| Deletion Size | 12,744 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 72,552,765 | 72,552,788 | ATGTGACCAGAGTTGTACTTCTTC |
| TUP | 72,552,728 | 72,552,756 | AGTATTCTCCCTGGAACCACTAGCCAAGG |
| TUR | 72,552,703 | 72,552,726 | CCCAGGTTCTTGTCGTCTTGATTC |
| TDF | 72,540,783 | 72,540,801 | GGGAGAGTGGCTGCCTATG |
| TDP | 72,540,747 | 72,540,776 | TGAGAAGCAGCAGAGAACTGGGAAGAACAT |
| TDR | 72,540,717 | 72,540,741 | GCCCTACCCAATAAACCCTATACAG |
PCR Primers
| Primer | Sequence |
| SU | AGTCATGCAGGAAACCAAGG |
| SD | CCATTCAAGATAGTCCATAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
