| Gene | Gabra4 |
| Chromosome | 5 |
| Deletion Start | 71,657,806 |
| Deletion End | 71,633,558 |
| Deletion Size | 24,249 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 71,657,643 | 71,657,664 | GGCAAGTGTCCAAATCCACAAC |
| TUP | 71,657,619 | 71,657,640 | CCCTCTGGCCACTCTTCCTGGA |
| TUR | 71,657,518 | 71,657,536 | TTCCTGCACCCGACTCTTC |
| TDF | 71,634,377 | 71,634,400 | TGTGATTGGATAGATTGCTCAGTG |
| TDP | 71,634,346 | 71,634,372 | CATCTGCCATGGTGGGAGGATAAGACC |
| TDR | 71,634,312 | 71,634,331 | GGCTCTTCCTGTGCATTCTG |
PCR Primers
| Primer | Sequence |
| SU | ACAAGGTGCGGAGCTGAG |
| SD | CAGGAATGTCACTGAGAGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 147 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13293A-A5 | VGB6 | C57BL/6NTac | |
| 13293A-F1 | VGB6 | C57BL/6NTac | |
