13294 Gba Status: Cancelled
| Gene | Gba |
| Chromosome | 3 |
| Deletion Start | 89,203,490 |
| Deletion End | 89,208,670 |
| Deletion Size | 5,181 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 89,203,493 | 89,203,513 | GCCAGGCTCATCGGATTCTTC |
| TUP | 89,203,518 | 89,203,543 | TTCAGGCGGTATCTTGGGCATATGGT |
| TUR | 89,203,556 | 89,203,577 | GACACCTTCATGCCTCCATCTC |
| TDF | 89,208,526 | 89,208,548 | GGATGTCCCTCTTACCATCAGTG |
| TDP | 89,208,550 | 89,208,572 | TCCTGACCTGGGCTTCCTGGAGA |
| TDR | 89,208,577 | 89,208,597 | TGAATGGAGTAGCCAGGTGAC |
PCR Primers
| Primer | Sequence |
| SU | GGGCTACAATTTGCCTTGTC |
| SD | GACTGACTGCCTTGCTCCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
