| Gene | Il2ra |
| Chromosome | 2 |
| Deletion Start | 11,674,300 |
| Deletion End | 11,689,869 |
| Deletion Size | 15,570 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 11,674,433 | 11,674,452 | GAAACTCCTGGAGCAGCAAC |
| TUP | 11,674,453 | 11,674,475 | TGCCAGTGCACCAGCAACTGTAA |
| TUR | 11,674,523 | 11,674,542 | TGGGAGCTGGAGTCTGTCAC |
| TDF | 11,689,394 | 11,689,414 | GCCCAGTAGGACATCTTGCTC |
| TDP | 11,689,417 | 11,689,442 | ACCCACTCAGCTCTATAACCGGCCTA |
| TDR | 11,689,443 | 11,689,467 | GGTCATCTCACAACACAACTAACTC |
PCR Primers
| Primer | Sequence |
| SU | CTGTAGAGGAACTTGAGTGAA |
| SD | ACTTGGGCTTTCCAAACTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 110 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13300A-E5 | VGB6 | C57BL/6NTac | |
| 13300A-A8 | VGB6 | C57BL/6NTac | |
| 13300A-A6 | VGB6 | C57BL/6NTac | |
| 13300A-H6 | VGB6 | C57BL/6NTac | |
| 13300A-H8 | VGB6 | C57BL/6NTac | |
| 13300A-H10 | VGB6 | C57BL/6NTac | |
