13317 Rpl12 Status: ES cell colonies screened / QC no positives
| Gene | Rpl12 |
| Chromosome | 2 |
| Deletion Start | 32,961,806 |
| Deletion End | 32,964,079 |
| Deletion Size | 2,274 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 32,962,165 | 32,962,186 | TGTTTGGCCTGTAGATGTGTTC |
| TUP | 32,962,198 | 32,962,221 | AGGGCAGAAGAGGCCACCATGTCC |
| TUR | 32,962,249 | 32,962,269 | TTTCCTTTGCGTCCCAAGTCC |
| TDF | 32,963,166 | 32,963,187 | GTCTGTTGTCTGTTCCCTTTCC |
| TDP | 32,963,192 | 32,963,217 | TGCAGAAATGAAACAGTGCCTCAGGG |
| TDR | 32,963,252 | 32,963,273 | AGTCCTCACCAGCAGCCCTAAC |
PCR Primers
| Primer | Sequence |
| SU | CTAGGGTCGCTCAAGTCAGG |
| SD | ACTGCTACTGCCATTGGTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 105 bp | + | + | - |
| TDF | TDR | 108 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
