13319 Scn2a1 Status: ES cell colonies screened / QC no positives
| Gene | Scn2a1 |
| Chromosome | 2 |
| Deletion Start | 65,670,499 |
| Deletion End | 65,690,328 |
| Deletion Size | 19,830 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 65,670,528 | 65,670,549 | TGACAGCTTCCGCTTCTTTACC |
| TUP | 65,670,557 | 65,670,584 | CCCTTGCTGCTATTGAACAACGCATTGC |
| TUR | 65,670,606 | 65,670,625 | CGTCCTTGCGTTCCTGTTTG |
| TDF | 65,690,189 | 65,690,209 | CAGAAAGAGCAGGCTGGAGAG |
| TDP | 65,690,216 | 65,690,242 | AAGGAAGATGCTGTGCGGAAATCTGCC |
| TDR | 65,690,285 | 65,690,306 | CGTAGGTCAGTCTACTCCCTTC |
PCR Primers
| Primer | Sequence |
| SU | ATCTTCCATTTGCAAGCTCC |
| SD | GTCTCAAGTCTTTAACAAAACCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 118 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
