| Gene | Htr1f |
| Chromosome | 16 |
| Deletion Start | 64,926,924 |
| Deletion End | 64,925,806 |
| Deletion Size | 1,119 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 64,926,584 | 64,926,605 | CGTGTTCCATCTTGCATCTGTC |
| TUP | 64,926,555 | 64,926,581 | CTATAGCCTTGGATAGGTACCGCGCCA |
| TUR | 64,926,510 | 64,926,529 | CATGCCTGGGAGTCCTCTTC |
| TDF | 64,926,295 | 64,926,314 | ACAGAGCAGCAAGGACACTG |
| TDP | 64,926,270 | 64,926,293 | ACCACAAGAGACAAGCGAGTCGGA |
| TDR | 64,926,227 | 64,926,248 | CCGCTCTCCAAGAAGACTTGAC |
PCR Primers
| Primer | Sequence |
| SU | GCATAGCATCTGCTGTAGAC |
| SD | TGGTTAAGCAATGAGGTGACAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 96 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13331A-A7 | VGB6 | C57BL/6NTac | |
| 13331A-E12 | VGB6 | C57BL/6NTac | |
