13339 Steap1 Status: Cancelled
| Gene | Steap1 |
| Chromosome | 5 |
| Deletion Start | 5,742,906 |
| Deletion End | 5,736,415 |
| Deletion Size | 6,492 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 5,742,023 | 5,742,041 | CCAGCATCGGAGCTTCTTC |
| TUP | 5,741,997 | 5,742,022 | TCAGCACACTTGGAGCTCTGAGAACG |
| TUR | 5,741,977 | 5,741,996 | TGGTGTTCCGTTTCCGTTTC |
| TDF | 5,736,510 | 5,736,534 | TCCTGATATGTAAAATCGCCCTGTG |
| TDP | 5,736,481 | 5,736,507 | TGCCCTGTCTGAGGAAGAAGATACTGA |
| TDR | 5,736,451 | 5,736,471 | TGCTGACATCTTCCCAACCAC |
PCR Primers
| Primer | Sequence |
| SU | ATGGTAGGTCATCCATTGGG |
| SD | ATGCATCATATCCACACACAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 65 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
