13350 Abhd1 Status: ES cell colonies screened / QC no positives
| Gene | Abhd1 |
| Chromosome | 5 |
| Deletion Start | 30,950,114 |
| Deletion End | 30,955,093 |
| Deletion Size | 4,980 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 30,950,963 | 30,950,983 | TTACTCCTGGCTACTGGGAAG |
| TUP | 30,950,988 | 30,951,014 | AGACAGGAGGATCACTTGAGCCCAAGA |
| TUR | 30,951,039 | 30,951,060 | GCACAGGGCCTTGTTATGTTAC |
| TDF | 30,954,204 | 30,954,228 | CCACTCAACTCACTGCTCTTCAATC |
| TDP | 30,954,229 | 30,954,252 | AGCCACTCACTGCTGGACTCTGCC |
| TDR | 30,954,266 | 30,954,286 | GCCTGCCATCTGCTATCCTAC |
PCR Primers
| Primer | Sequence |
| SU | ATGTCTGATTCAGCCTTCGC |
| SD | ATGTGAAGATGCCCAAGAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
