13351   Abhd11     Status: Cancelled

GeneAbhd11
Chromosome5
Deletion Start135,009,715
Deletion End135,012,115
Deletion Size2,401
Genome Build38
MGI IDMGI:1916008
NCBI Gene ID68758
BAC ID
BAC Library
CassetteZEN-Ub1
Clones AvailableNo

Primer Genomic Coordinates on Chromosome 5

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF135,009,776135,009,794GTTTGGCGGTTCCTGTCAC
TUP135,009,796135,009,819TTCTGTAGCAGCCGCAGTAGTGGC
TUR135,009,852135,009,874AGTGGAGAACAACCCTCCCAGTG
TDF135,011,184135,011,203TGCTCTGGTCTGATTCTGTC
TDP135,011,204135,011,227TCCCAGCCAGATGTTGTGGAACGA
TDR135,011,240135,011,259GTTCCTACCGGGCTTATGTC

PCR Primers

PrimerSequence
SUTGTTACCAAGCCAGCTAGTC
SDCCTCTGAGTCGGGAGTCTTG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR99 bp++-
TDFTDR76 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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