13354 Acad11 Status: ES cell colonies screened / QC no positives
| Gene | Acad11 |
| Chromosome | 9 |
| Deletion Start | 104,063,764 |
| Deletion End | 104,084,282 |
| Deletion Size | 20,519 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 104,063,832 | 104,063,853 | GGCCTACCTGAATCAGCACTTG |
| TUP | 104,063,854 | 104,063,876 | CCGGGCTTTGGGTCAGATTCCCG |
| TUR | 104,063,912 | 104,063,931 | CCCAGGAGGCAAAGGCTATG |
| TDF | 104,083,951 | 104,083,969 | TGCCTGCTGTTGGAAAGAC |
| TDP | 104,083,970 | 104,083,993 | TCTGAATTTGCTCCCTCGAGCTGC |
| TDR | 104,084,010 | 104,084,033 | GCCTTGGGCTTATTGTTCAGTTTG |
PCR Primers
| Primer | Sequence |
| SU | CGCGTCCTTAGTCTAGGGTG |
| SD | CCAAAGTTCACTGCCATGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
