13361 Zfp868 Status: ES cell colonies screened / QC one positive
| Gene | Zfp868 |
| Chromosome | 8 |
| Deletion Start | 69,619,349 |
| Deletion End | 69,611,413 |
| Deletion Size | 7,937 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 69,618,596 | 69,618,618 | CAGCAGTTCATAGATGGGAGTGG |
| TUP | 69,618,567 | 69,618,592 | AACTCAAAGTCCTGAAGCTGGGCCTG |
| TUR | 69,618,524 | 69,618,545 | CCAGTGCAGGAGAGCTATTGAG |
| TDF | 69,612,359 | 69,612,383 | TTTTCTCACAAGTGTTGAAGGCTAC |
| TDP | 69,612,332 | 69,612,357 | TGGAAACTCACACTGTGAATGGGCCT |
| TDR | 69,612,247 | 69,612,275 | CACACTCTTTGTACTCATAGTTGTGATGG |
PCR Primers
| Primer | Sequence |
| SU | AGGTAGACCTGAAAGTTGTG |
| SD | CCATGTGCTAGCCTTCTTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 137 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
