13364 Alkbh5 Status: ES cell colonies screened / QC no positives
| Gene | Alkbh5 |
| Chromosome | 11 |
| Deletion Start | 60,538,426 |
| Deletion End | 60,554,430 |
| Deletion Size | 16,005 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 60,539,067 | 60,539,088 | CATCGTGTCCGTGTCTTTCTTC |
| TUP | 60,539,091 | 60,539,114 | CGACTCGGCACTTTGCTTCGGCTG |
| TUR | 60,539,122 | 60,539,141 | ACCCGGATGGGCTTGAACTG |
| TDF | 60,553,784 | 60,553,804 | TCAGATCGCCTGTCAGGAAAC |
| TDP | 60,553,806 | 60,553,828 | CCAGAGACCCTGCGCTGAAACCC |
| TDR | 60,553,854 | 60,553,872 | TACCTGTGGGCAGCATCAG |
PCR Primers
| Primer | Sequence |
| SU | AGTACGTGGAGTCTCGTGGC |
| SD | CATCCAAGCATGAGCATCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
