13365 Ap2s1 Status: Cancelled
| Gene | Ap2s1 |
| Chromosome | 7 |
| Deletion Start | 17,323,786 |
| Deletion End | 17,334,359 |
| Deletion Size | 10,574 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 17,323,949 | 17,323,969 | GGGTTTCGGTTTGGAGGTGAC |
| TUP | 17,323,974 | 17,323,996 | TTCCTCCTGCTGCGGATGATCCG |
| TUR | 17,324,072 | 17,324,094 | CCAAATCCTTTGCTTGGAACCAC |
| TDF | 17,333,921 | 17,333,940 | GCACAGAGGACAGGCTTAGG |
| TDP | 17,333,947 | 17,333,974 | TTTCTCCTTTCACCATGGGATACAGGGT |
| TDR | 17,333,982 | 17,334,003 | CAGGTACAGACAAGGCCATCAG |
PCR Primers
| Primer | Sequence |
| SU | ATTTGGTACTCGCATTTGGC |
| SD | GCCCTGTGTAGGCAGAAGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 146 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
