13366 Apba3 Status: ES cell colonies screened / QC no positives
| Gene | Apba3 |
| Chromosome | 10 |
| Deletion Start | 81,268,898 |
| Deletion End | 81,273,127 |
| Deletion Size | 4,230 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 81,268,994 | 81,269,012 | AACACCCAGTCGGCCTTAG |
| TUP | 81,269,016 | 81,269,036 | CTGGAGGCCCTGTCACCCTGA |
| TUR | 81,269,039 | 81,269,059 | GGATGTGTCCAGCTCCATCTC |
| TDF | 81,273,016 | 81,273,038 | CCGTCTACCTGACTCTTCATCTC |
| TDP | 81,273,043 | 81,273,069 | CCCTAGATCCACATCAAGACCATGCCA |
| TDR | 81,273,088 | 81,273,107 | GGTTGTTCCTGTCCCGTGAG |
PCR Primers
| Primer | Sequence |
| SU | GGCTTCTGTGTAAAATGGGG |
| SD | CTCCACACTCCACATGGTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 66 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
