13367 Apoa2 Status: ES cell colonies screened / QC no positives
| Gene | Apoa2 |
| Chromosome | 1 |
| Deletion Start | 171,225,318 |
| Deletion End | 171,226,346 |
| Deletion Size | 1,029 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 171,225,661 | 171,225,685 | TGTAGGAGCTTTGGTTAAGAGACAG |
| TUP | 171,225,687 | 171,225,710 | CAGACGGACCGGATATGCAGAGCC |
| TUR | 171,225,757 | 171,225,777 | GAGGTCTTGGCCTTCTCCATC |
| TDF | 171,225,941 | 171,225,959 | AGATGAGGGCAGCAAGAGG |
| TDP | 171,225,960 | 171,225,982 | TGGACCTTGGACTCCAGTCTGCA |
| TDR | 171,225,985 | 171,226,004 | CATGCAGCAGAGGATCAGAC |
PCR Primers
| Primer | Sequence |
| SU | TTCTGGACTTGCCATTCTCC |
| SD | TGGGTCCATGTTCTTCAGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 117 bp | + | + | - |
| TDF | TDR | 64 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
