13368 Apoc4 Status: ES cell colonies screened / QC no positives
| Gene | Apoc4 |
| Chromosome | 7 |
| Deletion Start | 19,681,377 |
| Deletion End | 19,678,080 |
| Deletion Size | 3,298 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 19,680,561 | 19,680,581 | GGGTGCTGAGAACTGAAGAAC |
| TUP | 19,680,538 | 19,680,560 | TGGGTCCTCTGGAAGAGCACTGA |
| TUR | 19,680,513 | 19,680,534 | GGCACTGGCTGCTCATTAAGAG |
| TDF | 19,678,198 | 19,678,220 | CCAGAGACCACCTCCTAAACAAG |
| TDP | 19,678,175 | 19,678,197 | ACCCACAGCCTGTGTCCCAGACT |
| TDR | 19,678,137 | 19,678,157 | ACGTGGCACTTTAACCCTGAG |
PCR Primers
| Primer | Sequence |
| SU | CCCTCACATACACCACCAATC |
| SD | TATTGTGGAGGGCTTTCTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 69 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
