13369 Apom Status: ES cell colonies screened / QC positives
| Gene | Apom |
| Chromosome | 17 |
| Deletion Start | 35,268,714 |
| Deletion End | 35,266,058 |
| Deletion Size | 2,657 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 35,267,796 | 35,267,819 | CTGAAGGGAAAGGAAACATGGAAC |
| TUP | 35,267,770 | 35,267,795 | TCAGAACTGAAGGTTGGTTCCCACCA |
| TUR | 35,267,734 | 35,267,753 | GGGAAACGGCTAAGGGAGAC |
| TDF | 35,266,937 | 35,266,958 | GGCCAAAGGCAGACATTATCAG |
| TDP | 35,266,901 | 35,266,929 | ACACTGACCCCTCTAATTCTTATGGGCTA |
| TDR | 35,266,868 | 35,266,890 | CCTAACTGCACATGAGTCCTTCT |
PCR Primers
| Primer | Sequence |
| SU | AATTCCAGACTTGCCCAGTG |
| SD | GTGGGAGCCAGAAGAAACAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13369A-A3 | VGB6 | C57BL/6NTac | |
| 13369A-G7 | VGB6 | C57BL/6NTac | |
| 13369A-B5 | VGB6 | C57BL/6NTac | |
| 13369A-B4 | VGB6 | C57BL/6NTac | |
| 13369A-A6 | VGB6 | C57BL/6NTac | |
| 13369A-B6 | VGB6 | C57BL/6NTac | |
