13372 Arhgdig Status: ES cell colonies screened / QC positives
| Gene | Arhgdig |
| Chromosome | 17 |
| Deletion Start | 26,338,135 |
| Deletion End | 26,336,278 |
| Deletion Size | 1,858 |
| Genome Build | 37 |
|
|
Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 26,337,393 | 26,337,413 | GAGGAACAACCGCTGAGAGTG |
| TUP | 26,337,365 | 26,337,388 | TCCCAGCGCAGCACTTGAATGTTG |
| TUR | 26,337,311 | 26,337,330 | GAGGACTGCGAGTAGGGAAG |
| TDF | 26,336,388 | 26,336,408 | CAGTGGAGGAAGCACCAAGAG |
| TDP | 26,336,364 | 26,336,386 | AGCATTGGCACGTGGCCTCTACG |
| TDR | 26,336,312 | 26,336,331 | TCCCAGGACAGGTGGTTCAG |
PCR Primers
| Primer | Sequence |
| SU | AGGGAGCAGGTGGAGGGTT |
| SD | AGGGAGCACCTCTGAAGGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 103 bp | + | + | - |
| TDF | TDR | 97 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13372A-C3 | VGB6 | C57BL/6NTac | |
| 13372A-H7 | VGB6 | C57BL/6NTac | |
| 13372A-E9 | VGB6 | C57BL/6NTac | |
