| Gene | Asb3 |
| Chromosome | 11 |
| Deletion Start | 30,998,329 |
| Deletion End | 31,029,062 |
| Deletion Size | 30,734 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 30,998,964 | 30,998,983 | AGCTCTGGGTAGTCTGGAAC |
| TUP | 30,998,993 | 30,999,020 | TGCTTGCCTGTTTCATTTGAGTGCTAGG |
| TUR | 30,999,033 | 30,999,054 | AAAGAGGCTAGGCATGGTGGTG |
| TDF | 31,028,723 | 31,028,745 | TTGAGCATAGTTTCTGTGGCAAG |
| TDP | 31,028,749 | 31,028,774 | ACCAAAGGGCTGTTCACACTGGAGTC |
| TDR | 31,028,797 | 31,028,821 | TGAAACAGGAAAGTACCAGTGAGTC |
PCR Primers
| Primer | Sequence |
| SU | AATGTGTAGCCTGCTGGAAAG |
| SD | ATGATCCCTTCCATGGAACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13374A-G10 | VGB6 | C57BL/6NTac | |
| 13374A-D4 | VGB6 | C57BL/6NTac | |
