13381   Atp6v0c     Status: ES cell colonies screened / QC no positives

GeneAtp6v0c
Chromosome17
Deletion Start24,169,281
Deletion End24,164,400
Deletion Size4,860
Genome Build38
MGI IDMGI:88116
NCBI Gene ID11984
BAC ID428F1
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Primer Genomic Coordinates on Chromosome 17

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF24,168,68224,168,700TGGGTGAGGCAGGTGTTTC
TUP24,168,65724,168,681CGGTTGAGGCTTGTCTCGGTTTCCC
TUR24,168,63424,168,652CCCACACCGATGACTGAAC
TDF24,165,27024,165,292TCTAGGCAGGAACTGATTGACAC
TDP24,165,24424,165,266AGTGGCCTCTGGGCAGCTGACAT
TDR24,165,20224,165,221TCCCACACCCTAAGCTCCAG

PCR Primers

PrimerSequence
SUAACCCGTAGGGTTCGAGAAG
SDTCCATCCAAGGAGTCTCAGC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR67 bp++-
TDFTDR91 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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