13385 Avpr1a Status: Cancelled
| Gene | Avpr1a |
| Chromosome | 10 |
| Deletion Start | 122,448,808 |
| Deletion End | 122,452,365 |
| Deletion Size | 3,558 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 122,449,642 | 122,449,661 | TGTGTCAGCAGCGTGAAGAG |
| TUP | 122,449,664 | 122,449,687 | TTTCCCGTGCCAAGATCCGCACAG |
| TUR | 122,449,716 | 122,449,736 | GGTGTCCAGCAGAGGATGTAG |
| TDF | 122,452,210 | 122,452,228 | AGCGCAGAAATTCGCCAAG |
| TDP | 122,452,231 | 122,452,256 | TGACTCGGATAGCATGAGCCGGAGAC |
| TDR | 122,452,284 | 122,452,303 | CGTCCCAGTGCTGTTTGTTG |
PCR Primers
| Primer | Sequence |
| SU | AATCACCAACGACTTGGAGG |
| SD | TCACGGACCAGTTCACTTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 94 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
