13392 Bcl9l Status: Cancelled
| Gene | Bcl9l |
| Chromosome | 9 |
| Deletion Start | 44,499,527 |
| Deletion End | 44,509,843 |
| Deletion Size | 10,317 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 44,500,310 | 44,500,328 | CTCGGGCTTGAGCAGTGAC |
| TUP | 44,500,331 | 44,500,354 | ACAGGTCCTGTGGCCCTTCTCAGC |
| TUR | 44,500,360 | 44,500,379 | CAACTACGCTGTGCCCTGAC |
| TDF | 44,509,182 | 44,509,202 | GGGTGGCATCGGTACTGAATG |
| TDP | 44,509,203 | 44,509,227 | ACCCAGAGCTGAGTGAGGTGATCCG |
| TDR | 44,509,282 | 44,509,300 | GAGGGTGCTGCTTGGTTTC |
PCR Primers
| Primer | Sequence |
| SU | TTGCACGATACTGCAAGCTC |
| SD | GGATGGAAGACCCTCCTCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 119 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
