13393 Bik Status: ES cell colonies screened / QC no positives
| Gene | Bik |
| Chromosome | 15 |
| Deletion Start | 83,541,303 |
| Deletion End | 83,544,272 |
| Deletion Size | 2,970 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 83,541,757 | 83,541,777 | GGCGAGTCCTTCTGCACATAG |
| TUP | 83,541,779 | 83,541,803 | CAGGTCAGCACAGGCTCTTCACGTG |
| TUR | 83,541,836 | 83,541,855 | GGCGACACTGATTCCACAAC |
| TDF | 83,544,035 | 83,544,057 | GTTGTGCATAGGTGCTTCTTCTG |
| TDP | 83,544,065 | 83,544,086 | CCCAAGAGCTGGCTCCTCACCC |
| TDR | 83,544,107 | 83,544,127 | GGACCAAGAGGGAAAGGAGAG |
PCR Primers
| Primer | Sequence |
| SU | GTGTAGAGCTATGGCTGGGC |
| SD | TTGAACTTGCCTGCAAACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
