| Gene | Ccdc117 |
| Chromosome | 11 |
| Deletion Start | 5,542,055 |
| Deletion End | 5,531,240 |
| Deletion Size | 10,816 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 5,541,536 | 5,541,555 | TCACGGCCAAGGAAGGAATG |
| TUP | 5,541,511 | 5,541,535 | TTCAATTTGTGGCGGGAGATCCGGA |
| TUR | 5,541,486 | 5,541,507 | CACCATGTATTTGTCGCCTCAC |
| TDF | 5,531,509 | 5,531,530 | CCGTCCTTCTATGGAGCTTGTG |
| TDP | 5,531,480 | 5,531,505 | TGGAAACCTCTCCCTGAACTCCTTCC |
| TDR | 5,531,450 | 5,531,472 | TTAGGGCTAGATGATGGCTTTGG |
PCR Primers
| Primer | Sequence |
| SU | GACCTGACCTCCTGCAATTC |
| SD | AGTCCTGCTCTCTTCCCTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13407A-A8 | VGB6 | C57BL/6NTac | |
| 13407A-E3 | VGB6 | C57BL/6NTac | |
| 13407A-B10 | VGB6 | C57BL/6NTac | |
| 13407A-G4 | VGB6 | C57BL/6NTac | |
