13409 Ccdc15 Status: Cancelled
| Gene | Ccdc15 |
| Chromosome | 9 |
| Deletion Start | 37,348,063 |
| Deletion End | 37,320,404 |
| Deletion Size | 27,660 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 37,347,794 | 37,347,816 | TGAGAGGAACCAGGCCATAATAC |
| TUP | 37,347,762 | 37,347,784 | CTTGGGTGGAACCTGCCTCACCA |
| TUR | 37,347,707 | 37,347,727 | GATCACAGTAACCGGGTCAAC |
| TDF | 37,320,630 | 37,320,651 | GTCTCCTTCGCAAGTAATCCTC |
| TDP | | | TGTCCGAAGTCTGGC |
| TDR | 37,320,563 | 37,320,580 | CCAAGCGCACTGCTGTTC |
PCR Primers
| Primer | Sequence |
| SU | TCAACAACCCGCTCTAGTCC |
| SD | AGTTTTCCCTAATTTTCAGGTAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 110 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
