13415 Ccne1 Status: ES cell colonies screened / QC no positives
| Gene | Ccne1 |
| Chromosome | 7 |
| Deletion Start | 38,106,797 |
| Deletion End | 38,098,504 |
| Deletion Size | 8,294 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 38,105,907 | 38,105,930 | CAACTCTTTCTCCCTTGTCCATTC |
| TUP | 38,105,869 | 38,105,898 | TTTCCTAATCCCAGCAGTAGAGAAGCAAGG |
| TUR | 38,105,822 | 38,105,841 | GGGTCTCACTCTACGGTCTG |
| TDF | 38,099,292 | 38,099,310 | GGTGTCCTCGCTGCTTCTG |
| TDP | 38,099,256 | 38,099,285 | ATCATTTCTCCTCACTGGAGTTGATGCAGA |
| TDR | 38,099,223 | 38,099,246 | AACTCACAGGAAAGCCCAACATAC |
PCR Primers
| Primer | Sequence |
| SU | AAAGGGACACTGAGGCAGC |
| SD | AACCTACAACACCCGAGCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 109 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
