13418 Cd3d Status: ES cell colonies screened / QC no positives
| Gene | Cd3d |
| Chromosome | 9 |
| Deletion Start | 44,981,889 |
| Deletion End | 44,986,346 |
| Deletion Size | 4,458 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 44,982,781 | 44,982,803 | GAAGGCTGGCTGTAGACATAAGG |
| TUP | 44,982,804 | 44,982,824 | CATGGCAGTGCCCTCTGGGCT |
| TUR | 44,982,849 | 44,982,868 | TCTGGTGGGAGAGGTGAAAG |
| TDF | 44,985,655 | 44,985,675 | TCATTGACCTCATCGCAACTC |
| TDP | 44,985,676 | 44,985,697 | TGCTCCTGGCTTTGGGCGTCTA |
| TDR | 44,985,701 | 44,985,721 | CCGGTCTCATGTCCTGCAAAG |
PCR Primers
| Primer | Sequence |
| SU | GCTTCAGAAAGAGCAGTTTGC |
| SD | CTGCTTTGGGCTGTGGTAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 67 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
