13419   Cd63     Status: ES cell clone microinjected

GeneCd63
Chromosome10
Deletion Start128,910,409
Deletion End128,912,841
Deletion Size2,433
Genome Build38
MGI IDMGI:99529
NCBI Gene ID12512
BAC ID373D17
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 10

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF128,911,203128,911,226AGCTGGGAGTCCATAGAAAGTAAC
TUP128,911,227128,911,250CCATATCCTGTGCCGAAGCGCTGT
TUR128,911,264128,911,284AGAAGGGAATCGGGAGGTGTC
TDF128,912,604128,912,623GTGGCTGTTGGATGGGTTTG
TDP128,912,627128,912,649AAGCTTGCCCTGCCTCCTCTCCA
TDR128,912,660128,912,683TCCCAAGACCTGAAAGGAAAGATG

PCR Primers

PrimerSequence
SUAGATCTACCTGGAGTCCGGC
SDGCCCTCAAAGCATCAGAAAG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR82 bp++-
TDFTDR80 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
13419A-A5VGB6C57BL/6NTac
13419A-A6VGB6C57BL/6NTac
13419A-A8VGB6C57BL/6NTac
13419A-B1VGB6C57BL/6NTac
13419A-E4VGB6C57BL/6NTac
13419A-G6VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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