13423 Ift46 Status: Targeting vector QC completed
| Gene | Ift46 |
| Chromosome | 9 |
| Deletion Start | 44,586,683 |
| Deletion End | 44,598,715 |
| Deletion Size | 12,033 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 44,586,760 | 44,586,778 | TCTGGGTGTGGTCAGTGTG |
| TUP | 44,586,781 | 44,586,803 | AAGGCTCTGAGTCCGCAGGAACC |
| TUR | 44,586,845 | 44,586,868 | TGCCATCATCTCAAGACTCTACTC |
| TDF | 44,598,236 | 44,598,257 | CACGGCTTTGTGTTTGAAGGAG |
| TDP | 44,598,259 | 44,598,280 | TGGGCGCATTGCTGACCCTTCC |
| TDR | 44,598,291 | 44,598,313 | AGGCTTCCAGGCTCACACTTTAG |
PCR Primers
| Primer | Sequence |
| SU | AAGGTGCAAACATATTTGTTTTT |
| SD | GGGAGAAAGGGAGGTGAAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 109 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
