13428 Ckb Status: ES cell colonies screened / QC no positives
Gene | Ckb |
Chromosome | 12 |
Deletion Start | 111,671,955 |
Deletion End | 111,669,502 |
Deletion Size | 2,454 |
Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
Primer | Start | End | Sequence |
TUF | 111,671,050 | 111,671,071 | CCTGTCCTTGAACTCTGCACTC |
TUP | 111,671,023 | 111,671,047 | CAGCTCTGTCCAGCCTAGATGGCGA |
TUR | 111,670,979 | 111,670,998 | CCGCCTCAGTCATGCTCTTG |
TDF | 111,670,436 | 111,670,456 | AGCTGCCTTCCTCCACTGTTG |
TDP | 111,670,406 | 111,670,429 | TTGGCGTCTTGGTGACAGCTCACA |
TDR | 111,670,309 | 111,670,327 | TGGAGTCCCTGCCAGAATG |
PCR Primers
Primer | Sequence |
SU | TGCAAGCACAGGCATCCATCG |
SD | AGATGACACAGAAGGCCAGC |
LacInR | TTGACTGTAGCGGCTGATGTTG |
LacInf | GGTAAACTGGCTCGGATTAGGG |
NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
NeoInF | TTCGGCTATGACTGGGCACAACAG |
LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
Forward Primer | Reverse Primer | Length | WT | HET | KO |
SU | LacZRev | bp | - | + | + |
TUF | TUR | 93 bp | + | + | - |
TDF | TDR | 148 bp | + | + | - |
NeoFwd | SD | bp | - | + | + |
LacInf | LacInR | 210 bp | - | + | + |
NeoInf | NeoInR | 282 bp | - | + | + |